HLA Class 1 Antigen B12 (FITC) Antibody

  • Catalog number
    GEN600362
  • Price
    Please ask
  • Size
    0.25 ml
  • Also known as
    HLA Class 1 Antigen B12
  • Other names
    HLA class I histocompatibility antigen, B-40 alpha chain; HLA class I histocompatibility antigen, B-7 alpha chain; major histocompatibility complex, class I, B; lymphocyte antigen; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC class I antigen GN00104; leukocyte antigen class I-B; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen HLA-B heavy chain; HLA class I histocompatibility antigen, B alpha chain; major histocompatibility complex, class I, B; MHC class I antigen B*7
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    HLA-B; HLA-B; AS; HLAB; SPDA1; HLAB
  • Gene name synonims
    HLA-B; HLA-B; AS; HLAB; SPDA1; HLAB
  • Other gene names
    HLA-B; HLA-B; AS; HLAB; SPDA1; HLAB
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG2b
  • Clone
    0.L.4
  • Host organism
    Mouse (Mus musculus)
  • Source organism
    Human (Homo sapiens)
  • Species reactivity
    Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    Reacts to HLA Class 1 Antigen-B12 when used at a dilution of 1:10 when using the microcytoxicity test under standard NIH conditions.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    Highly Purified by Ion Exchange chromatography.
  • Form Appearance
    Supplied as a liquid in PBS, 0.1% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (FITC).
  • Concentration
    N/A
  • Storage and shipping
    Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. Please contact MyBioSource for bulk requirements and licensing requests relating to production applications.
  • Tested applications
    Flow Cytometry (FC/FACS), Immunofluorescence (IF)
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
  • Description
    Antigens are peptides or recombinant or native dependent on the production method.
  • Conjugation
    Anti-FITC Antibody
  • French translation
    anticorps
  • Gene target
    HLA   Class   B12  
  • Gene symbol
    NDUFB3, HLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DQA2, HLA-DPB1, HLA-DQB2, HLA-W, HLA-DRA
  • Short name
    HLA Class 1 Antigen B12 (FITC) Antibody
  • Technique
    Antibody, antigen, FITC, antibodies against human proteins, antibodies for, antigenes, Fluorescein
  • Label
    FITC
  • Alternative name
    Anti-HLA Class 1 Antigen B12 (FITC)
  • Alternative technique
    antibodies, antigenes, fluorescine
Gene info
  • Identity
    HGNC:7698
  • Gene
    NDUFB3
  • Long gene name
    NADH:ubiquinone oxidoreductase subunit B3
  • Synonyms gene name
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3 (12kD, B12)
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa
  • Synonyms
  • Synonyms name
  • GenBank acession
  • Locus
    2q33.1
  • Discovery year
    1997-12-17
  • Entrez gene record
    4709
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • NADH:ubiquinone oxidoreductase supernumerary subunits
  • VEGA ID
    OTTHUMG00000132820
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
    Fluorescent Antibody Technique
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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