Anti-DYKDDDDK (FLAG) Tag (FG4R) Monoclonal Antibody
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Catalog number
GEN525006
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Price
Please ask
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Size
1000ug
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Also known as
FLAG Tag
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
FG4R
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Host organism
Mouse (Mus musculus)
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Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein A affinity chromatography from mouse ascites fluid
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Form Appearance
N/A
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Concentration
N/A
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Storage and shipping
Store the antibody should be stored at -20 degrees Celsius. for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at Store the antibody at +4 degrees Celsius. (add 0.05% NaN3 if not included) for several days to weeks
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Tested applications
Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Short name
Anti-DYKDDDDK ( ) Tag (FG4R) Monoclonal Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
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Label
Flag
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Alternative name
Anti-Anti-DYKDDDDK (FLAG) Tag (FG4R) Monoclonal Antibody
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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