Phospho-N-WASP(Tyr256) Antibody

Type

primary antibody
Shipping

Ice bag
Antigen

Phospho-N-WASP(Tyr256)
Reactivity

Human,Mouse,Rat
Clonality

Polyclonal
Application

WB,IHC,ELISA
Application_advice

WB 1:500-1:2000, IHC 1:50-1:200
Epitope

Phospho Tyr256
Immunogen

A synthesized peptide derived from human N-WASP around the phosphorylation site of Tyr256.
Purification

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Format

Liquid
Concentration

1mg/ml
Fragment

Fab fragment
Storage_Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Specificity

Phospho-N-WASP(Tyr256) Antibody detects endogenous levels of N-WASP only when phosphorylated at Tyr256.
GendID

8976
Gene

WASL
GeneAlias

WASL
Unigene

Hs.143728;Hs.731519;Hs.83916;
Swiss Prot

O00401
Weight

54kDa
Datasheet
Msds
Properties

If you buy Antibodies supplied by Affinity Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Identity

HGNC:12735
Gene

WASL
Long gene name

WASP like actin nucleation promoting factor
Synonyms gene name
- Wiskott-Aldrich syndrome-like
- Wiskott-Aldrich syndrome like
Synonyms
- N-WASP
- NWASP
- WASPB
GenBank acession
- D88460
Locus

7q31.32
Discovery year

1998-10-14
Entrez gene record

8976
Pubmed identfication
- 9422512
- 9322739
- 23527602
RefSeq identity
- NM_003941
Classification
- Wiskott-Aldrich Syndrome protein family
VEGA ID

OTTHUMG00000157346

Identity

HGNC:12731
Gene

WAS
Long gene name

WASP actin nucleation promoting factor
Synonyms gene
- IMD2
- THC
Synonyms gene name
- thrombocytopenia 1 (X-linked)
- Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
- Wiskott-Aldrich syndrome
Synonyms
- WASP
- WASPA
Synonyms name
- eczema-thrombocytopenia
GenBank acession
- AF115548
Locus

Xp11.23
Discovery year

2001-06-22
Entrez gene record

7454
Pubmed identfication
- 7795648
- 23527602
RefSeq identity
- NM_000377
Classification
- Wiskott-Aldrich Syndrome protein family
VEGA ID

OTTHUMG00000034483
Locus Specific Databases
- WASbase: Mutation registry for Wiskott-Aldrich syndrome (WAS)
- LRG_125

Identity

HGNC:7989
Gene

NRAS
Long gene name

NRAS proto-oncogene, GTPase
Synonyms gene name
- neuroblastoma RAS viral (v-ras) oncogene homolog
- neuroblastoma RAS viral oncogene homolog
Synonyms
- N-ras
GenBank acession
- BC005219
Locus

1p13.2
Discovery year

2001-06-22
Entrez gene record

4893
Pubmed identfication
- 6705568
- 11524732
RefSeq identity
- NM_002524
Classification
- RAS type GTPase family
VEGA ID

OTTHUMG00000012059
Locus Specific Databases
- NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IV
- LRG_92

Identity

HGNC:16660
Gene

FERD3L
Long gene name

Fer3 like bHLH transcription factor
Synonyms gene name
- Fer3-like (Drosophila)
- Fer3-like bHLH transcription factor
Synonyms
- NATO3
- N-TWIST
- bHLHa31
GenBank acession
- AF369897
Locus

7p21.1
Discovery year

2002-12-09
Entrez gene record

222894
Pubmed identfication
- 11472856
- 12217327
Classification
- Basic helix-loop-helix proteins
VEGA ID

OTTHUMG00000090823

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data