Caspase-1 Substrate YVAD-AFC

• Catalog number
  1103-1000
• Price
  Please ask
• Size
  1000 assays

• Alternative_name
  Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic
• Description
  Ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80), and DTT used for caspase assays are also available separately.
• Structure Available
  No
• Peptide sequence
  Ac-Tyr-Val-Ala-Asp-AFC
• Salt Form
  No
• Molecular Formula
  C₃₃H₃₆F₃N₅O₁₀
• Molecular Weight
  719.7
• Cell Permeable
  No
• Purity
  >99% by HPLC
• Handling
  Protect from light and moisture
• Tag Line
  A caspase-1 substrate
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• MDL Number
  MFCD01318881
• PubChem CID
  44135090
• PubChem CID Link
• SMILES
  CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C
• InChi
  InChI=1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)
• InChi Key
  GWKJISJKNKIJNP-UHFFFAOYSA-N
• Data Sheet Link
• MSDS Available
  Yes
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

• Gene target
  Caspase-1   Substrate   YVAD-AFC  
• Short name
  Caspase-1 Substrate YVAD-AFC
• Alternative name
  caspase-1 Substrate YVAD-AFC

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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