FITC Donkey Anti-Goat IgG (H+L) Antibody

• Catalog number
  MBS128205
• Price
  Please ask
• Size
  0.1 mL

• Products_type
  Secondary Antibody
• Products_short_name
  [IgG (H+L)]
• Products_name_syn
  [FITC Donkey Anti-Goat IgG (H+L): N/A]
• Purity
  Affinity Purification
• Form
  Fluorescein
• Concentration
  1mg/ml
• Storage_stability
  Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Tested_application
  Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Latin name
  Equus africanus asinus, Capra aegagrus hircus
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
• French translation
  anticorps

• Gene target
  H+L  
• Gene symbol
  CALD1, FUT1, L3MBTL2
• Short name
  FITC Anti-Goat IgG (H+L) Antibody
• Technique
  Antibody, anti-, anti, FITC, Goat, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, goats, IgGs
• Host
  Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgG, IgG
• Label
  FITC
• Species
  Donkeys, Goats
• Alternative name
  fluorecein Donkey Antibody toGoat Immunoglobulin G (heavy + light chain) (antibody to-)
• Alternative technique
  antibodies, caprine, fluorescine, immunoglobulins

Gene info

• Identity
  HGNC:1441
• Gene
  CALD1
• Long gene name
  caldesmon 1
• Synonyms
  • CDM
  • H-CAD
  • L-CAD
• GenBank acession
  • M64110
• Locus
  7q33
• Discovery year
  1992-07-20
• Entrez gene record
  800
• Pubmed identfication
  • 1885618
• RefSeq identity
  • NM_033138
• VEGA ID
  OTTHUMG00000155407

Gene info

• Identity
  HGNC:4012
• Gene
  FUT1
• Long gene name
  fucosyltransferase 1 (H blood group)
• Synonyms gene
  • H
  • HSC
• Synonyms gene name
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
• Synonyms name
  • galactoside 2-alpha-L-fucosyltransferase
• Locus
  19q13.33
• Discovery year
  1989-06-06
• Entrez gene record
  2523
• RefSeq identity
  • NM_000148
• Classification
  • Fucosyltransferases
  • Blood group antigens
• VEGA ID
  OTTHUMG00000183325
• Locus Specific Databases
  • Blood Group Antigen Mutation Database
  • LRG_810

Gene info

• Identity
  HGNC:18594
• Gene
  L3MBTL2
• Long gene name
  L3MBTL histone methyl-lysine binding protein 2
• Synonyms gene name
  • l(3)mbt-like 2 (Drosophila)
  • L3MBTL2, polycomb repressive complex 1 subunit
• Synonyms
  • H-l(3)mbt-l
  • DKFZP761I141
  • dJ756G23.3
• GenBank acession
  • AJ305226
• Locus
  22q13.2
• Discovery year
  2002-04-29
• Entrez gene record
  83746
• Pubmed identfication
  • 11682070
  • 21596310
• RefSeq identity
  • NM_031488
• Classification
  • MBT domain containing
• VEGA ID
  OTTHUMG00000150942

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products