Thrombin, Active, Human Plasma

• Catalog number
  7593-250
• Price
  Please ask
• Size
  250 U

• Synonyms
  Activated Factor IIa
• Alternative_names
  Activated Factor IIa
• Description
  A coagulation protein and a serine protease.
• Recombinant
  No
• Source
  Human Plasma
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  >3000 units/mg protein.
• Results
  >3000 units/mg protein.
• Unit Definition
  Activity is expressed in NIH/US units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. The NIH assay procedure uses 0.2 ml of diluted plasma (1:1 with saline) as a Substrate and 0.1 ml of albumin solution based on a modification of the method of Biggs. Only clotting times in the range of 15–25 seconds are used for determining thrombin activity. Optimal clotting temperature is 37°C. There are approximately 5 Thrombin cleavage units for every one US Unit.
• Molecular Weight
  37 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from a buffer composed of 20 mM Bis-Tris, 150 mM NaCl and 0.1% PEG 8000, pH 6.5.
• Reconstitution Instructions
  A working stock solution can be prepared by adding 1 mL buffered saline, pH 7.4 (ex. 25 mM HEPES, 150 mM NaCl, 0.1% PEG 8000, pH 7.4). Further dilution should be made in buffer containing a suitable stabilizing agent such as 0.1%-1% Prionex, BSA, or PEG.
• Background Information
  Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  Thrombin   Active   Plasma  
• Short name
  Thrombin, Active, Plasma
• Species
  Human, Humans
• Alternative name
  Thrombin, functionnal, H. sapiens Plasma

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