Thrombin, Active, Bovine Plasma (High Activity)

  • Catalog number
    7592-100
  • Price
    Please ask
  • Size
    100 KU
  • Synonyms
    Activated Factor IIa
  • Alternative_names
    Activated Factor IIa
  • Description
    A coagulation protein and a serine protease.
  • Recombinant
    No
  • Source
    Bovine plasma
  • Purity by SDS PAGE
    ≥98%
  • Assay
    SDS-PAGE
  • Biological activity
    >1500 units/mg powder.
  • Results
    >1500 units/mg powder.
  • Unit Definition
    Activity is expressed in NIH/US units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. The NIH assay procedure uses 0.2 ml of diluted plasma (1:1 with saline) as a Substrate and 0.1 ml of albumin solution based on a modification of the method of Biggs. Only clotting times in the range of 15–25 seconds are used for determining thrombin activity. Optimal clotting temperature is 37°C. There are approximately 5 Thrombin cleavage units for every one US Unit.
  • Molecular Weight
    37 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Sterile filtered and lyophilized with Mannitol and Sodium Chloride.
  • Reconstitution Instructions
    Reconstitute in sterile water (100 U/ml) with 0.9% NaCl. It forms a clear solution.
  • Background Information
    Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Gene target
  • Short name
    Thrombin, Active, Plasma ( Activity)
  • Species
    Bovine, Bos Taurus genes are available form the Bovine Genome database.
  • Alternative name
    Thrombin, functionnal, Bovine Plasma (High Activity)
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