Thrombin, Active, Bovine Plasma (Technical grade)

• Catalog number
  7591-10
• Price
  Please ask
• Size
  10 KU

• Synonyms
  Activated Factor IIa
• Alternative_names
  Activated Factor IIa
• Description
  A coagulation protein and a serine protease.
• Recombinant
  No
• Source
  Bovine plasma
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Biological activity
  90-250 U/mg of lyophilized powder.
• Results
  90-250 U/mg of lyophilized powder.
• Unit Definition
  Activity is expressed in NIH/US units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. The NIH assay procedure uses 0.2 ml of diluted plasma (1:1 with saline) as a Substrate and 0.1 ml of albumin solution based on a modification of the method of Biggs. Only clotting times in the range of 15–25 seconds are used for determining thrombin activity. Optimal clotting temperature is 37°C. There are approximately 5 Thrombin cleavage units for every one US Unit.
• Molecular Weight
  37 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Sterile filtered and lyophilized with Mannitol and Sodium Chloride.
• Reconstitution Instructions
  Reconstitute in sterile water (100 U/ml) with 0.9% NaCl. It forms a clear solution.
• Background Information
  Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet

• Gene target
  Thrombin   Active   Plasma   Technical   grade  
• Short name
  Thrombin, Active, Plasma (Technical grade)
• Species
  Bovine, Bos Taurus genes are available form the Bovine Genome database.
• Alternative name
  Thrombin, functionnal, Bovine Plasma (Technical grade)

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