Human Recombinant Hexokinase 1

• Catalog number
  6309-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant Hexokinase 1
• Alternative_names
  Hexokinase type I, Brain form hexokinase, HK1.
• Description
  An enzyme that phosphorylates hexoses
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is 7-8 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP.
• Unit Definition
  In the coupled mode, one unit will produce 1.0 µmole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25°C.
• Molecular Weight
  104.6 kDa
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
• Background Information
  Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV This enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Hexokinase  
• Gene symbol
  HK2P1, HK1, HKDC1
• Short name
  Recombinant Hexokinase 1
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. Hexokinase 1
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4924
• Gene
  HK2P1
• Long gene name
  hexokinase 2 pseudogene 1
• Synonyms gene
  • HK2P
• Synonyms gene name
  • hexokinase 2 pseudogene
• Locus
  Xq21.1
• Discovery year
  1995-05-26
• Entrez gene record
  642546
• Pubmed identfication
  • 7999069
  • 28618048
• RefSeq identity
  • NG_009860
• VEGA ID
  OTTHUMG00000021906

Gene info

• Identity
  HGNC:4922
• Gene
  HK1
• Long gene name
  hexokinase 1
• GenBank acession
  • M75126
• Locus
  10q22.1
• Discovery year
  2001-06-22
• Entrez gene record
  3098
• RefSeq identity
  • NM_000188
• VEGA ID
  OTTHUMG00000018380
• Locus Specific Databases
  • LRG_365

Gene info

• Identity
  HGNC:23302
• Gene
  HKDC1
• Long gene name
  hexokinase domain containing 1
• Synonyms
  • FLJ37767
  • FLJ22761
• Locus
  10q22.1
• Discovery year
  2004-05-27
• Entrez gene record
  80201
• Pubmed identfication
  • 25648650
  • 27459389
• RefSeq identity
  • NM_025130
• VEGA ID
  OTTHUMG00000018371

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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