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Category
Secondary Antibodies
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Long description
The reactivity of the antiserum is directed to the Fc subunit of subclass IgG2. It does not react with other IgG1, IgG/Fab fragments, IgM and IgA or any non-Ig protein in bovine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2 in bovine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of bovine IgG2 from serum or plasma.
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Antibody come from
Pools of purified normal IgG2 isolated from pooled bovine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Bovine IgG2 (subclass specific) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Bovine
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Antibody s reacts with these species
This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.
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Antibody s specificity
Precipitating polyclonal Rabbit antiSerum to Bovine IgG2, subclass specific.
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Research interest
Veterinary
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Application
Immunoprecipitation
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Antibody s suited for
Immunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less then 1:32 when tested against pooled normal bovine serum in agar block immunodiffusion titration.
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Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
