anti-EGLN3 Antibody
-
Catalog number30-813
-
PricePlease ask
-
Size50 ug
-
-
Gene infoEGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. EGLN3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. EGLN3 promotes cell death through a caspase-dependent mechanism.
-
Gene namesEGLN3, FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3, HIFP4H3
-
Antibodie s labelunlabelled serum
-
PurityAntibody is purified by peptide affinity chromatography method.
-
Ab raised inRabbit
-
TypePolyclonal
-
AntigenAntibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN3.
-
Immuno applicationELISA, Western Blot
-
Antibody adviceEGLN3 antibody can be used for detection of EGLN3 by ELISA at 1:1562500. EGLN3 antibody can be used for detection of EGLN3 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
-
Suspention pH azideAntibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
-
Keep atFor short periods of storage (days) store at 4 grades C. For longer periods of storage, store EGLN3 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
-
Works withHuman, Mouse, Rat
-
Omim nr11545787
-
Ncbi nrNP_071356
-
KDa27 kDa
-
Vial with antibodyLyophilized
-
Quantity per ml1 mg/ml
-
NCBI GENE nr112399
-
GENE symbolEGLN3
-
Antigene nameegl-9 family hypoxia-inducible factor 3
-
SourceHomo sapiens
-
Protein nrQ9H6Z9
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolEGLN3-AS1, EGLN3
-
Short nameanti-EGLN3 Antibody
-
TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
-
Alternative nameAntibody toEGLN3 (antibody to-)
-
Alternative techniqueantibodies
-
Alternative to gene targetegl nine homolog 3 (C. elegans), EGLN3 and IDBG-4515 and ENSG00000129521 and 102724945,112399, oxidoreductase activity, nuclei, Egln3 and IDBG-139438 and ENSMUSG00000035105 and 112407, EGLN3 and IDBG-632414 and ENSBTAG00000008172 and 535578
-
Gene info
-
Identity
-
Gene
-
Long gene nameEGLN3 antisense RNA 1
-
GenBank acession
-
Locus
-
Discovery year2013-08-23
-
Classification
- Antisense RNAs
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameegl-9 family hypoxia inducible factor 3
-
Synonyms gene name
- egl nine homolog 3 (C. elegans)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-08-21
-
Entrez gene record
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data