ApoJ/Clusterin, human recombinant

• Catalog number
  7584-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, ApoJ
• Alternative_names
  CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, ApoJ
• Description
  A heavily glycosylated protein upregulated in multiple pathological conditions
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  The chaperone activity of ApoJ was confirmed by its ability to bind to proteins under physical stress such as heat (e.g. Thyroglobulin) and chemical stress such as change in pH (e.g. Procathepsin K).
• Biological activity
  The chaperone activity of ApoJ was confirmed by its ability to bind to proteins under physical stress such as heat (e.g. Thyroglobulin) and chemical stress such as change in pH (e.g. Procathepsin K).
• Molecular Weight
  51.1 kDa (23-449 aa with a C-terminal His-tag)
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 2 mg/ml solution of ApoJ in 25 mM Na2HPO4 and 100 mM NaCl (pH 7.5).
• Reconstitution Instructions
  Reconstitute in dH2O to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely.
• Background Information
  Native Apolipoprotein J (ApoJ), also named Clusterin, is a heavily glycosylated, 75-80 kDa disulfide-linked heterodimeric protein. Despite being cloned since 1989, no genuine function has been attributed to ApoJ so far. The protein has been reportedly implicated in several diverse physiological processes such as sperm maturation, lipid transportation, complement inhibition, tissue remodeling, membrane recycling, cell-cell and cell-substratum interactions, stabilization of stressed proteins in a folding-competent state and promotion or inhibition of apoptosis. ApoJ gene is differentially regulated by cytokines, growth factors and stress-inducing agents. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ApoJ/Clusterin  
• Gene symbol
  CLU
• Short name
  ApoJ/Clusterin, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  ApoJ/Clusterin, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:2095
• Gene
  CLU
• Long gene name
  clusterin
• Synonyms gene
  • CLI
  • APOJ
• Synonyms gene name
  • clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)
• Synonyms
  • SGP-2
  • SP-40
  • TRPM-2
  • KUB1
  • CLU1
  • CLU2
• Synonyms name
  • complement lysis inhibitor
  • sulfated glycoprotein 2
  • testosterone-repressed prostate message 2
  • apolipoprotein J
• GenBank acession
  • M64722
• Locus
  8p21.1
• Discovery year
  1990-07-26
• Entrez gene record
  1191
• Pubmed identfication
  • 1585460
• RefSeq identity
  • NM_001831
• Classification
  • Complement system regulators and receptors
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000102114

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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