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HIF 1 Alpha Antibody ALEXA FLUOR

HIF 1 Alpha Antibody ALEXA FLUOR is available 5 times from Bioss primary conjugated antibodies. alexa fluor labs

bs-0737R-A350 | HIF-1 Alpha Antibody, ALEXA FLUOR 350 size: 100ul | 429.06 USD

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bs-0737R-A488 | HIF-1 Alpha Antibody, ALEXA FLUOR 488 size: 100ul | 429.06 USD

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bs-0737R-A555 | HIF-1 Alpha Antibody, ALEXA FLUOR 555 size: 100ul | 429.06 USD

Catalog number bs-0737R-A555
Price 429.06 USD
Size 100ul
Long name HIF-1 Alpha Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
Also known as Anti-HIF-1 Alpha PAb ALEXA FLUOR 555
Conjugated with ALEXA FLUOR® 555
Excitation emission 553nm/568nm
Properties For facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation Alexa Fluor,ALEXA FLUOR 555
Description The Anti-HIF-1 Alpha is a α- or alpha protein sometimes glycoprotein present in blood.
French translation anticorps
Label ALEXA FLUOR 555
Alternative name HIF-1 Alpha Polyclonal Antibody, ALEXA FLUOR 555
1. Gene info
Identity 700
Gene ARNT
Long gene name aryl hydrocarbon receptor nuclear translocator
Synonyms
  • HIF-1beta
  • bHLHe2
GenBank acession
  • AF001307
Locus 1q21.3
Discovery year 1990-01-22
Entrez gene record 405
Classification
  • Basic helix-loop-helix proteins
Havana BLAST/BLAT OTTHUMG00000035011
2. Gene info
Identity 4910
Gene HIF1A
Long gene name hypoxia inducible factor 1 subunit alpha
Synonyms gene name
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms
  • MOP1
  • HIF-1alpha
  • PASD8
  • HIF1
  • bHLHe78
GenBank acession
  • U22431
Locus 14q23.2
Discovery year 1995-03-27
Entrez gene record 3091
Pubmed identfication
  • 8786149
  • 9079689
RefSeq identity
  • NM_001530
Classification
  • Basic helix-loop-helix proteins
Havana BLAST/BLAT OTTHUMG00000140344
3. Gene info
Identity 3374
Gene EPAS1
Long gene name endothelial PAS domain protein 1
Synonyms
  • MOP2
  • PASD2
  • HIF2A
  • HLF
  • bHLHe73
Synonyms name
  • HIF-1 alpha-like factor
GenBank acession
  • U81984
Locus 2p21
Discovery year 1998-05-29
Entrez gene record 2034
Pubmed identfication
  • 9000051
  • 9079689
  • 18378852
RefSeq identity
  • NM_001430
Classification
  • Basic helix-loop-helix proteins
Havana BLAST/BLAT OTTHUMG00000128818
Product images
    #.png#bs#.jpgFormalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody (bs-0737R), Unconjugated  at 1:300, followed by conjugation to the secondary antibody and DAB stainingL1 mouse heart lysates, L2 mouse large intestine lysates probed with Anti HIF-1 Alpha Polyclonal Antibody, Unconjugated (bs-0737R) at 1:200 in 4?C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37?C. Predicted band aKLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-0737R) followed by a FITC Conjugated Secondary Antibody.Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. Rat brain and spleen lysates probed with Rabbit Anti-HIF-1 Alpha Polyclonal Antibody (bs-0737R) at 1:1000.HL-60 cell lysates probed with Anti-HIF-1 Alpha Polyclonal Antibody, Unconjugated (bs-0737R) at 1:300 overnight at 4?C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37?C.HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated (bs-0737R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Product files

bs-0737R-A594 | HIF-1 Alpha Antibody, ALEXA FLUOR 594 size: 100ul | 429.06 USD

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bs-0737R-A647 | HIF-1 Alpha Antibody, ALEXA FLUOR 647 size: 100ul | 429.06 USD

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Category Conjugated Primary Antibodies
Host Organism Rabbit (Oryctolagus cuniculus)
Target Antigen HIF-1 Alpha
Specificity This is a highly specific antibody against HIF-1 Alpha.
Modification Unmodified
Modification Site None
Clonality Polyclonal
Clone Polyclonal antibody
Concentration 1ug per 1ul
Immunogen range 351-400/826
Subcellular location Cytoplasm, Nucleus
Source This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human of human HIF-1 Alpha
Gene ID Number 3091
Swiss Prot Q16665
Tested applications IF(IHC-P), IF(ICC)
Recommended dilutions IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
Crossreactivity Human, Mouse, Rat
Cross-reactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 4 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP3. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Purification Purified by Protein A.
Storage conditions Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Synonyms HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A
Gene targetHIF-1 Alpha
Short name Anti-HIF-1 Alpha
Technique Antibody
Isotype Immunoglobulin G (IgG)
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Anti-HIF-1 Alpha | Technique alternative | 01010441510
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