Synthetic Bombesin (frog) conjugated with bovine thyroglobulin
Bombesin (pGlu-Gln-Arg-Leu-Gly Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2) and related peptides e.g. Gastrin Releasing Peptide (mammalian homolog aa sequence bold)
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
Product is supplied as
Recommended concentration for use
Amphibian brain, gut (peripheral nerves) fetal lung
No protease pre-treatment required. Improvement may be achieved by HIER.
Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.
Go to UniProt/SwissProt
1. Costa M., Furness J.B., Yanaihara N., Yanaihara C., and Moody T.W. (1984) Cell and Tissue res. 235; 285-293 2. Gomi T., Kikuchi Y., Adriensen D., Timmermans J.-P., De Groodt-Lassel M.H.A. et al. (1994) Immunocytochemical survey of the neuroepithelial endocrine system in the respiratory tract of the Tokyo salamander, Hynobis nebulosus tokyoensis TAGO. Histochemistry 102; 425-431.
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.