pVision-RFP-C Vector
-
Catalog number9996-20
-
PricePlease ask
-
Size20 μg
-
-
DescriptionAn eukaryotic expression vector encoding red fluorescent protein. pVisionRFP-C is an eukaryotic (mammalian) expression vector encoding red fluorescent protein VisionRFP from sea anemone Entacmaea quadricolor. The vector allows to generate fusions to the VisionRFP C-terminus and to express VisionRFP fusions or VisionRFP alone in eukaryotic (mammalian) cells. pVisionRFP-C vector carries synthetic VisionRFP gene which codon usage is humanized, i.e. optimized for high expression in mammalian cells. pVisionRFP-C vector contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication and neomycin resistance (Neor) gene for selection in eukaryotic cells. It also contains pUC origin of replication for propagation in E. coli and f1 origin for single-stranded DNA production. Bacterial promoter (P) provides expression of kanamycin resistance gene in E. coli. To increase VisionRFP translation, Kozak consensus translation initiation site is generated upstream of VisionRFP sequence. Multiple cloning site (MCS) is between VisionRFP coding sequence and the SV40 polyadenylation signal (SV40 poly A).
-
Product Highlights• Amount - 20 µg • Application: C-terminal mammalian expression vector encoding humanized TagRFP and allowing TagRFP expression and generation of fusions to the TagRFP C-terminus. Generation of pVisionRFP-fusion proteins- A localization signal or a gene of interest should be inserted into MCS of the vector. It will be expressed as a fusion to VisionRFP C-terminus when inserted in the same reading frame as VisionRFP and no intervening stop codons are present. Note: Despite its dimeric structure, VisionRFP is still suitable for generation of fusions with proteins of interest. Expression in mammalian cells- pVisionRFP-C vector can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of VisionRFP or VisionRFP-tagged fusions in many cell types. If required, stable transformants can be selected using G418. Propagation in E. coli - Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue. -Selectable marker: plasmid confers resistance to kanamycin (30 µg/ml) to E. coli hosts. - E. coli replication origin: pUC - Copy number: ~500 -Plasmid incompatibility group: pMB1/ColE1
-
Storage Temp-20°C
-
Shippinggel pack
-
Shelf Life12 months
-
NotesFor research use only. Not to be used for human or animal treatment or consumption.
-
Gene target
-
Gene symbolTRIM27, SMC4, PCK1, MYB, SFTPC, VCX3B, MAF, RFX1, EGR4
-
Short namepVision-RFP-C Vector
-
TechniqueVectors
-
Alternative namepVision-Red Fluorescent-C integrating Desoxyribonucleic acid sequence
-
Gene info
-
Identity
-
Gene
-
Long gene nametripartite motif containing 27
-
Synonyms gene
-
Synonyms gene name
- ret finger protein
- tripartite motif-containing 27
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1995-05-09
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Ring finger proteins
- Tripartite motif containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namestructural maintenance of chromosomes 4
-
Synonyms gene
-
Synonyms gene name
- SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
- SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-02-22
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Structural maintenance of chromosomes proteins
- Condensin I subunits
- Condensin II subunits
- MicroRNA protein coding host genes
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namephosphoenolpyruvate carboxykinase 1
-
Synonyms gene name
- phosphoenolpyruvate carboxykinase 1 (soluble)
-
Synonyms
-
Locus
-
Discovery year1990-09-10
-
Entrez gene record
-
Pubmed identfication
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameMYB proto-oncogene, transcription factor
-
Synonyms gene name
- v-myb avian myeloblastosis viral oncogene homolog
-
Synonyms
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Myb/SANT domain containing
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namesurfactant protein C
-
Synonyms gene
-
Synonyms gene name
- surfactant, pulmonary-associated protein C
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1988-05-11
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- BRICHOS domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namevariable charge X-linked 3B
-
Synonyms
-
Locus
-
Discovery year2005-01-11
-
Classification
- Variable charge X/Y family
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameMAF bZIP transcription factor
-
Synonyms gene name
- v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
-
Synonyms
-
Locus
-
Discovery year1991-08-01
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Basic leucine zipper proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameregulatory factor X1
-
Synonyms gene name
- regulatory factor X, 1 (influences HLA class II expression)
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1991-11-06
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Regulatory factor X family
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameearly growth response 4
-
Synonyms
-
Locus
-
Discovery year1992-07-27
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Zinc fingers C2H2-type
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
-
Tree numbers
- E05.393.350.800
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
