Water-Soluble Tetrazolium Cell Proliferation Assay Kit

• Catalog number
  X2833K
• Price
  Please ask
• Size
  500 Tests

• Category
  Other Reagents
• Long description
  WST-8 Cell Proliferation Kits allows very convenient assays by utilizing ExalphaÕs highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H- tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator, as shown in Figure 1._x000B__x000B_WST-8 is a one-bottle solution; no premixing of components is required. WST-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium (Figure 2). The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells._x000B__x000B_Figure 3 shows that the cell proliferation assay using WST-8 correlates well with the [3H]-thymidine incorporation assay. Thus, the WST-8 assay can also be substituted for the [3H]-thymidine incorporation assay. As shown in Figure 4, the detection sensitivity using WST-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
• Antibody come from
  n/a
• Other description
  No other information available
• Clone
  not specified
• Antigen antibody binding interaction
  Water-Soluble Tetrazolium Cell Proliferation Assay Kit
• Antibody is raised in
  see techfile
• Antibody s reacts with
  see techfile
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  not tested yet / to be tested
• Antibody s suited for
  contact us
• Storage
  -20ºC
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Test
  nordc supplies other types of Assays as 1.
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Water-Soluble   Tetrazolium   Proliferation   Kit  
• Short name
  Water-Soluble Tetrazolium Proliferation Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  Water-Soluble Tetrazolium cellular expantion test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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