Rabbit Anti-Human CLK1 (pSer337 + pThr338) Polyclonal: RPE
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Catalog numberSPC-952D-RPE
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PricePlease ask
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Size100 µl
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ImmunogenA phospho-specific peptide corresponding to residues surrounding Ser337 and Thr338 of human CLK1 (AA334-341)
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Antibody s targetHuman CLK1 (pSer337 + pThr338)
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Antibody s full descriptionRabbit Anti-Human CLK1 (pSer337 + pThr338) Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameCDC like kinase 1 Antibody, CDC28/CDC2 like kinase Antibody, CLK Antibody, CLK/STY Antibody, CLK1_HUMAN Antibody, Protein tyrosine kinase STY Antibody, STY Antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 57.205 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-952 was sufficient for detection of CLK1 (pSer337 + pThr338) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellNucleus
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextThe CDC-like kinase 1 (CLK1) dually phosphorylates serine- and arginine-rich proteins of the spliceosomal complex, which constitutes a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Specifically, CLK1 may mediate the release of specific proteins from nuclear storage sites. Expression of CLK1 may be very low due to a premature stop codon in the mRNA, which leads to nonsense-mediated mRNA decay. CLK1 activity is positively regulated by phosphorylation on either tyrosine residues or serine/thre-onine residues. CLK1 activity is negatively regulated by steric constraints mediated by the N-terminal domain and also by phosphorylation on a subset of serine/threonine residues within the catalytic domain.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_001155879
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Gene number1195
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Protein numberP49759
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolRPE, CLK1
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Short nameRabbit Anti- CLK1 (pSer337 + pThr338) Polyclonal: RPE
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelRPE
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman CLK1 (pSer337 + pThr338) polyclonal: RPE
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCDC like kinase 1
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Synonyms gene name
- CDC-like kinase 1
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GenBank acession
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Locus
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Discovery year1991-08-07
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Entrez gene record
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Pubmed identfication
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Classification
- CDC like kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data