anti-sIL-2Ra Antibody [SB-647]
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Catalog number
38-230
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Price
Please ask
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Size
0.5 mg
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Gene names
Interleukin-2 receptor subunit alpha, TAC antigen, IL-2 receptor subunit alpha, CD25, IL2R, TCGFR, IDDM10
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Antibodie s label
unlabelled serum
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Purity
This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
SB-647
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Antigen
sIL-2Ra antibody is produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen.
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Immuno application
ELISA, Western Blot, NELISAut
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Antibody advice
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Ra; (10.0 ug/mL), a concentration of 1.0-1.5 ug/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 ug/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Ra; when used in conjunction with compatible secondary reagents. Western Blot:To detect hsIL-2Ra; by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Ra; is 2.0-4.0 ng/lane, under reducing or non-reducing conditions
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Keep at
sIL-2Ra lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 grades C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4 grades C when reconstituted.
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Works with
Human
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Omim nr
124317
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Ncbi nr
P01589
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Vial with antibody
Lyophilized
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NCBI GENE nr
3559
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GENE symbol
IL2RA
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Antigene name
interleukin 2 receptor, alpha
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Source
Homo sapiens
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Protein nr
P01589
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
MIR647
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Short name
anti-sIL-2Ra Antibody [SB-647]
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody tosIL-2Ra (antibody to-) [SB-647]
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
microRNA 647
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2006-05-17
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Entrez gene record
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RefSeq identity
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Classification
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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