Mouse Anti-Rat VGlut3 Monoclonal IgG1, Clone S34-34
-
Catalog numberSMC-397D-A390
-
PricePlease ask
-
Size100 µg
-
-
CloneS34-34
-
ImmunogenFusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGlut3
-
Antibody s full descriptionMouse Anti-Rat VGLUT3 Monoclonal IgG1 Antibody, Clone: S34-34: ATTO 390
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameDFNA25 Antibody, Vesicular glutamate transporter 3 Antibody, deafness autosomal dominant 25 Antibody, SLC17A8 Antibody, Solute carrier family 17 member 8 Antibody, VGLUT 3 Antibody, Solute carrier family 17 (sodium dependent inorganic phosphate cotransporter) member 8 Antibody
-
Raised inMouse
-
Antibody s targetVGLUT3
-
Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers, Pumps/Transporters, Neurotransmitter Transporters
-
BrandnameNone
-
Antibodies applicationsWB, IHC, ICC/IF
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~65kDa.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-397 was sufficient for detection of VGLut3 in 20 µg of CV-1 fibroblast cells (lysate) transfected with VGlut3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellCytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Membrane , Cell Junction , Synapse
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextVesicular Glutamate Transporter 3 (VGLUT3) is a multipass membrane protein restricted to synaptic vesicles of glutamergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord, and thalamus. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.
-
Bibliography1. Amilhon B., et al. (2010) J Neurosci. 30(6): 2198-2210. 2. Herzog E., et al. (2004) Neurosci. 123(4): 983-1002.
-
Released date16-May-2012
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_714947.1
-
Gene number266767
-
Protein numberQ7TSF2
-
Antibody s datasheetContact our support service
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGlut3 Monoclonal Antibody, Clone S34-34 (SMC-397). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGlut3 Monoclonal Antibody (SMC-397) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VGlut3 Antibody (D) Composite. Mouse Anti-VGlut3 Antibody [S34-34] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-397)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus, Rattus norvegicus
-
Gene target
-
Gene symbolSNORD115-34, IGKV3D-34, IGHV4-34, IGKV3-34, IGLV2-34, IGHV7-34-1, RNU6-34P, RASSF2, PHF11, IL34
-
Short nameMouse Anti- VGlut3 Monoclonal IgG1, Clone S34-34
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG1, IgG1
-
LabelATTO 390
-
SpeciesRat, Mouses, Rats
-
Alternative nameMouse Antibody toRat VGlut3 monoclonal IgG1, clonality S34-34
-
Alternative techniquemurine, antibodies
-
Clone nameS34-34
-
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-34
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 3D-34 (pseudogene)
-
Synonyms gene name
- immunoglobulin kappa variable 3D-34
- immunoglobulin kappa variable 3D-34 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 4-34
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 3-34 (pseudogene)
-
Synonyms gene name
- immunoglobulin kappa variable 3-34
- immunoglobulin kappa variable 3-34 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 2-34 (pseudogene)
-
Synonyms gene name
- immunoglobulin lambda variable 2-34
- immunoglobulin lambda variable 2-34 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 7-34-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable 7-34-1
- immunoglobulin heavy variable 7-34-1 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 34, pseudogene
-
Synonyms gene
-
Synonyms gene name
- RNA, U6 small nuclear 34
-
Locus
-
Discovery year2008-06-10
-
Entrez gene record
-
RefSeq identity
Gene info
-
Identity
-
Gene
-
Long gene nameRas association domain family member 2
-
Synonyms gene name
- Ras association (RalGDS/AF-6) domain family member 2
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2000-08-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Ras association domain family
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namePHD finger protein 11
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2003-05-08
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- PHD finger proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 34
-
Synonyms gene
-
Synonyms gene name
- chromosome 16 open reading frame 77
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2006-08-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data