Akt(Protein kinase B, PKB) (1D1) Monoclonal Antibody

• Catalog number
  bsm-50299M
• Price
  Please ask
• Size
  0.1ml

• Long name
  Akt(Protein kinase B, PKB) (1D1) Monoclonal Antibody
• Also known as
  Akt(Protein kinase B, PKB) (1D1) Monoclonal Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  Akt(Protein kinase B, PKB)
• Specificity
  This is a highly specific antibody against Akt(Protein kinase B, PKB).
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Monoclonal
• Clone number
  1D1
• Source
  Synthetic peptide
• Gene ID number
  207
• Swiss Prot
  P31749
• Tested Applications
  WB, IP
• Recommended dilutions
  WB (1:1,000) IP (1 ul)
• Cross reactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Akt (protein kinase B, PKB) is a serine/threonine protein kinase. Akt is activated by PI3 kinase, which is itself activated by tyrosine kinase (Thr308, Ser473). Akt/PKB controls vital cellular functions such as cell survival/apoptosis, cell cycle progression and glucose metabolism. It also acts down-stream from growth factors and hormones. Akt/PKB can inhibit cell cycle arrest by phosphorylating p21. Also, Akt/PKB may potentiate cell cycle progression through increased translation of cyclin D. Akt/PKB directly affects the apoptosis pathway by, for example, targeting the pro-apoptotic Bcl-2 related protein, BAD. It also can phosphorylate three kinases upstream of SAPK. The SAPK system consists of two groups of kinases, and JNK and p38 MAP kinase pathways. The SAPK pathway is an important target for Akt/PKB regulation to promote cell survival. Akt/PKB is able to regulate cell survival through transcriptional factors(Forkhead, nuclear factor-kB(NF-kB), Murine double minute 2(Mdm2)) that are responsible for pro- as well as anti-apoptotic genes. One of the first physiological targets of PKB to be identified was glycogen synthase kinase-3(GSK3), which has been associated with the control of many cellular processes, including glycogen and protein synthesis, and the modulation of transcription factor activity. The involvement of Akt in disease processes like malignancy, neurodegeneration and abnormal glucose metabolism has also been demonstrated.
• Purification method
  Ammonium sulfate precipitation
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  Akt   Protein   kinase   PKB   1D1   Monoclonal  
• Gene symbol
  AKT1
• Short name
  Anti-Akt(Protein kinase B, PKB)
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  Immunoglobulin G (IgG)1 λ
• Alternative name
  Akt(Protein kinase B, PKB) (1D1) Monoclonal Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:391
• Gene
  AKT1
• Long gene name
  AKT serine/threonine kinase 1
• Synonyms gene name
  • v-akt murine thymoma viral oncogene homolog 1
• Synonyms
  • RAC
  • PKB
  • PRKBA
  • AKT
  • RAC-alpha
• Synonyms name
  • protein kinase B
• GenBank acession
  • M63167
• Locus
  14q32.33
• Discovery year
  1986-01-01
• Entrez gene record
  207
• Pubmed identfication
  • 21214427
• RefSeq identity
  • NM_005163
• Classification
  • Pleckstrin homology domain containing
  • AKT kinases
• VEGA ID
  OTTHUMG00000170795
• Locus Specific Databases
  • LRG_721

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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