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Also known as
Cytokeratin 18, Caspase-cleaved
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Other names
cytokeratin 18; Keratin, type I cytoskeletal 18; keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein; keratin 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
KRT18; KRT18; K18; CYK18; CYK18
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Gene name synonims
KRT18; KRT18; K18; CYK18; CYK18
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Other gene names
KRT18; KRT18; K18; CYK18; CYK18
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2b
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Clone
6D45
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Bovine, Human (Homo sapiens), Monkey; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes the human CK18-Asp396 neo-epitope of caspase-cleaved CK18. Species Crossreactivity: monkey and bovine. Does not crossreact with intact CK18.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified supernatant
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Form Appearance
Supplied as a liquid in PBS, 0.1% BSA, PEG, sucrose and 0.09% sodium azide. Labeled with biotin.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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French translation
anticorps
