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Also known as
Cytokeratin 18, Caspase-cleaved
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Other names
cytokeratin 18; Keratin, type I cytoskeletal 18; keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein; keratin 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
KRT18; KRT18; K18; CYK18; CYK18
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Gene name synonims
KRT18; KRT18; K18; CYK18; CYK18
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Other gene names
KRT18; KRT18; K18; CYK18; CYK18
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2b
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Clone
6D45
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Bovine, Human (Homo sapiens), Monkey; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes the human CK18-Asp396 neo-epitope of caspase-cleaved CK18. Species Crossreactivity: monkey and bovine. Does not crossreact with intact CK18.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified supernatant
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Form Appearance
Supplied as a liquid in PBS containing 0.1% BSA, PEG, sucrose and 0.09% sodium azide.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Long-term storage should be done after aliquoting the antibody and freezing the aliqotes at -20 degrees Celsius. Under these conditions the aliquots will be stable for at least one year if kept at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
