Thrombin Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K374-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple assay to screen thrombin inhibitors
• Summary
  • Detection Method – Fluorescence (Ex/Em = 350/450 nm) • Application: - Screening potential inhibitors of thrombin - Characterize/study thrombin inhibitors
• Detection Method
  Fluorescence (Ex/Em = 350/450 nm)
• Applications
  • Screening potential inhibitors of thrombin • Characterize/study thrombin inhibitors
• Features Benefits
  • Rapid & simple • Reliable test for screening potential inhibitors of thrombin • Includes thrombin Inhibitor [PPACK Dihydrochloride]
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Thrombin enzyme (Factor IIa) is an important clotting factor that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin inhibitors are used as anticoagulants to prevent arterial and venous thrombosis. Some of these inhibitors are currently in clinical use while others are in clinical development. Biovision’s Thrombin Inhibitor Screening Kit utilizes the ability of Thrombin to cleave a synthetic AMC-based peptide substrate to release AMC, which can be detected by measuring its fluorescence at Ex/Em = 350/450 nm. In the presence of Thrombin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The kit provides a simple and rapid method to screen potential inhibitors of Thrombin.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Thrombin   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  Thrombin Inhibitor Screening Kit (Fluorometric)
• Alternative name
  Thrombin suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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