Human CellExp™ SERPIN F1, human recombinant

  • Catalog number
    7418-50
  • Price
    Please ask
  • Size
    50 ug
  • Synonyms
    SERPINF1, Serpin F1, PEDF, PIG35, EPC-1
  • Alternative_names
    SERPINF1, Serpin F1, PEDF, PIG35, EPC-1
  • Description
    Has a variety of prpperties including antiangiogenic, antitumorigenic, and neurotrophic properties
  • Recombinant
    Yes
  • Source
    HEK293 cells
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is typically 0.5-1.6 µg/ml.
  • Results
    Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is typically 0.5-1.6 µg/ml.
  • Molecular Weight
    This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45.2 kDa. The predicted N-terminus is Gln 20. DTT-reduced Protein migrates as 45-55 kDa.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
  • Background Information
    Serpin F1 (SERPINF1) is also known as Pigment epithelium-derived factor (PEDF), Cell proliferation-inducing gene 35 protein (PIG35). Serpin F1 belongs to the serpin family. Serpin F1 is expressed in quiescent cells. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. Endothelial cell migration is inhibited by SERPINF1/ PEDF. PEDF / SERPINF1 suppresses retinal neovascularization and endothelial cell proliferation. PEDF is also responsible for apoptosis of endothelial cells either through the p38 MAPK pathway or through the FAS/FASL pathway. PEDF also displays neurotrophic functions.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    CellExp™ SERPIN F1, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ SERPIN F1, H. sapiens Rec.
  • Alternative technique
    rec
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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