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Category
Primary Antibodies
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Long description
Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated α and β subunits. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. CD104 is a 205kD type I transmembrane glycoprotein, also known as beta 4 integrin. CD104 assoaciates with CD49f (integrin alpha 6) forming a heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation.
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Antibody come from
58XB4 is a monoclonal antibody obtained by immunization of Balb/c mice with Integrin β4. The immunogen used to raise this antibody was the full length CD104 protein.
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Other description
Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
58XB4
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Antigen antibody binding interaction
Mouse anti Integrin beta 4 (CD104) Antibody
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Antibody is raised in
Mouse (Balb/c)
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
Binds specifically to integrin β4 chain(CD104) or β4 integrin, that associates with integrin α6 (CD49f) forms α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the prolifeRative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells.
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Research interest
Cell adhesion,Immunology, CD Markers
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Application
ELISA,Flow Cytometry,Immunofluorescence,Immunohistochemistry (frozen),Immunoprecipitation,Western blotting,Immunocytochemistry
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Antibody s suited for
58XB4 is useful for Enzyme Linked Immuno Sorbent Assays (ELISA), Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western blotting, immunocytochemistry of paraformaldehyde fixed cells and Immubohistochemistry on frozen sections. For protocols see reference 1. Optimal antibody dilution should be determined by titration.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
Dirk Geerts, Lionel Fontao, Mirjam G. Nievers, Roel Q.J. Schaapveld, Patricia E. Purkis, Grant N. Wheeler, E. Birgitte Lane, Irene M. Leigh, and Arnoud Sonnenberg. J Cell Biol. 1999 October 18; 147(2): 417–434.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
