Active SIRT6 (GST-tagged), human recombinant
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Catalog number7697-100
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PricePlease ask
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Size100 µg
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SynonymsSIRT6, NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6
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Alternative_namesSIRT6, NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6
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DescriptionGST tagged Human SIRT6 from E.Coli, AOF, ≥85% pure, biologically active.
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RecombinantYes
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SourceE. coli.
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Purity by SDS PAGE≥85%
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AssaySDS-PAGE
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Activity Specifications test methodBioVision’s Active GST-tagged SIRT6 has been tested for its activity using Sirtuin Activity Assay Kit (K324-100). It has a specific activity of >3 mU/mg (One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of AFC/min at 37°C).
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Biological activity>3 mU/mg
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Results>3 mU/mg
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Unit DefinitionOne unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of AFC/min at 37°C
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Molecular WeightHuman SIRT6 is a 65.3 kDa (1-355 aa + N-terminal GST tag) protein. It runs as ~62 kDa on a SDS-PAGE.
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Storage Temp-80°C
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Shippingdry ice
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form descriptionHuman SIRT6 protein is a solution of 1 mg/ml in 50 mM Tris, 100 mM NaCl, pH 8.0 containing 20% glycerol.
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Background InformationHuman Sirtuins function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Sirtuin 6 (SIRT6) is a NAD-dependent protein deacetylase with activity towards Lys-9 and Lys-56 of histone H3. It modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It is required for genomic stability and for normal IGF1 serum levels and normal glucose homeostasis. It also regulates cellular senescence and apoptosis. Unlike most sirtuins, SIRT6 preferentially removes long-chain fatty-acyl, rather than acetyl, protein modifications. This activity regulates secretion of the inflammation-associated protein TNF-α.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMGST3, SIRT6
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Short nameActive SIRT6 (GST-tagged), recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namefunctionnal SIRT6 (GST-tagged), H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesirtuin 6
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Synonyms gene name
- sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
- sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
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GenBank acession
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Locus
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Discovery year2001-03-20
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Entrez gene record
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Pubmed identfication
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Classification
- Sirtuins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data