CaspSCREEN™ Flow Cytometric Apoptosis Detection Kit

• Catalog number
  K200-100
• Price
  Please ask
• Size
  100 assays

• Description
  Measures Activated Caspases in intact cells by FACS
• Summary
  • Detection method- Flow cytometry, L-1 channel (Ex/Em = 488/530 nm). • Species reactivity- Mammalian • Kit size- Convenient sizes (25 and 100 assays) • Applications- Detection of activated caspases in living cells.
• Sample Type
  Live cells
• Features Benefits
  • Simple one-step procedure; takes only 30 minutes • Fast and convenient • D₂R is more cell-permeable than other fluorometric caspase substrates
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  6-12 months
• Background
  Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D₂R), a reported substrate for members of caspase family proteases. The caspase substrate D₂R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.
• Data Sheet Link
• Additional description
  This 1 is suited for programmed cell-death studies. The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

• Gene target
  CaspSCREEN™   Cytometric   Kit  
• Short name
  CaspSCREEN™ Cytometric Apoptosis Kit
• Technique
  apoptosis, detection, Flow, apoptosis pathway
• Alternative name
  CaspSCREEN™ Flow Cytometric programmed cellular destruction quantification reagent
• Alternative technique
  celldeath, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products