Donkey anti Goat IgG H + L FITC
-
Catalog number
43C-CB0516
-
Price
Please ask
-
Size
1 mg
-
-
Category
Secondary Antibody
-
Antibody Subtype
Conjugated Secondary Antibodies
-
Area of research
Immunology
-
Type of Immunogen
Donkey anti-goat IgG (H+L) (FITC) was raised in donkey using goat IgG, whole molecule ..
-
Raised in
Donkey
-
Specificity
Goat IgG (H + L)
-
Cross Reactivity
Should be determined by the end user
-
Conjugate Tag
FITC
-
Method of Purification
Donkey anti Goat IgG (H + L) (FITC) was purified by affinity chromatography.
-
Form Buffer
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
-
Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditions
Blue Ice
-
Tested for
ELISA; FC; IF
-
Usage Recommendations
ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,0001:5,000
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Latin name
Equus africanus asinus, Capra aegagrus hircus
-
Properties
This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
-
Gene target
-
-
Gene symbol
CALD1, FUT1, L3MBTL2
-
Short name
anti Goat IgG H + L FITC
-
Technique
anti, FITC, Goat, IgG, antibody to, Fluorescein, goats, IgGs
-
Host
Goat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Isotype
IgG, IgG
-
Label
FITC
-
Species
Donkeys, Goats
-
Alternative name
Donkey anti Goat IgG (H + L) secondary antibody (FITC)
-
Alternative technique
antibodies, caprine, fluorescine, immunoglobulins
-
Gene info
-
Identity
-
Gene
-
Long gene name
caldesmon 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1992-07-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products