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Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr179 and Ser180 of human IKKa (AA177-184)
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Antibody s target
Human IKKa (pThr179+pSer180)
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Antibody s full description
Rabbit Anti-Human IKKa (pThr179+pSer180) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Chuk Antibody, CHUK1 Antibody, Conserved Helix Loop Helix Ubiquitous Kinase Antibody, I Kappa B Kinase 1 Antibody, IkB kinase alpha subunit Antibody, IkBKA Antibody, IKK 1 Antibody, IKK A Antibody, IKKA Antibody, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells Antibody, NFKBIKA Antibody, Nuclear Factor Kappa B Inhibitor Kinase Alpha Antibody, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor Antibody, TCF16 Antibody, Transcription factor 16 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 84.654 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-998 was sufficient for detection of IKKa (pThr179+pSer180) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Widely expressed.
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Scientific context
IKKa is a protein-serine/threonine kinase. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Loss of function therefore blunts the NF-kB response. Cells with DNA damage are unable to undergo apoptosis. Oncogenesis may therefore occur indirectly by inhibitting a downstream tumour suppresor (inhibitors of NF-kB).
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_001269
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Gene number
1147
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Protein number
O15111
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus