PARP-1 (D214) polyclonal antibody
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Catalog numberBS7047-50ul
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PricePlease ask
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Size50ul
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Alternative NamePoly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; PARP1; ADPRT; PPOL
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ImmunogenSynthetic peptide, corresponding to amino acids 180-214 of Human PARP-1.
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Swiss ProtP09874
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ReactivityHuman,Mouse,Rat
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ApplicationsWB
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Application_allWB: 1:500~1:1000
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ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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SpecificityPARP-1 (D214) polyclonal antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage.
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UsageWestern blot (WB) analysis of PARP-1 (D214) polyclonal antibody at 1:1000 dilution Lane1:NIH-3T3 whole cell lysate(20ug) Lane2:NIH-3T3 treated with PMA (60ng/ml, 5min) whole cell lysate (20ug) Lane3:NIH-3T3 treated with PMA (60ng/ml, 15min) whole cell lysate (20ug) Lane4:NIH-3T3 treated with PMA (60ng/ml, 30min) whole cell lysate (20ug)
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BiowMW~ 89, 116 kDa
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NoteFor research use only, not for use in diagnostic procedure.
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PropertiesIf you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Gene symbolPARP1, TIPARP, TNKS2, PARP12, TNKS, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1
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Short namePARP-1 (D214) polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit
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Alternative namePARP-1 (D214) polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene namepoly(ADP-ribose) polymerase 1
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Synonyms gene
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Synonyms gene name
- ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
- poly (ADP-ribose) polymerase family, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers PARP-type
- Zinc fingers
- Poly(ADP-ribose) polymerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTCDD inducible poly(ADP-ribose) polymerase
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Synonyms gene name
- TCDD-inducible poly(ADP-ribose) polymerase
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametankyrase 2
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Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-05-10
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Entrez gene record
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepoly(ADP-ribose) polymerase family member 12
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Synonyms gene
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Synonyms gene name
- zinc finger CCCH-type domain containing 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Zinc fingers CCCH-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametankyrase
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Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data