Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal: PerCP

  • Catalog number
    SPC-994D-PCP
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    A phospho-specific peptide corresponding to residues surrounding Thr187 of human MAP4K4 (AA184-190)
  • Antibody s target
    Human MAP4K4 (pThr187)
  • Antibody s full description
    Rabbit Anti-Human MAP4K4 (pThr187) Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Hepatocyte progenitor kinase like/germinal center kinase like kinase Antibody, HGK Antibody, HPK/GCK like kinase Antibody, KIAA0687 Antibody, M4K4_HUMAN Antibody, MAP4K4 Antibody, MAPK/ERK kinase kinase kinase 4 Antibody, MEK kinase kinase 4 Antibody, MEKKK 4 Antibody, Mitogen activated protein kinase kinase kinase kinase 4 Antibody, Nck interacting kinase Antibody, NIK Antibody
  • Verified applications
    WB, AM
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:250); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.025% Thimerosal
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects 142.101 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:250 dilution of SPC-994 was sufficient for detection of MAP4K4 (pThr187) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
  • Scientific context
    MAP4K4 appears to act upstream of the JUN N-terminal pathway. It phosphorylates SMAD1 on Thr-322.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_001155032
  • Gene number
    9448
  • Protein number
    O95819
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    No Data Available
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugate
    Peridinin chlorophyll (PerCP)
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    MAP4K4   pThr187  
  • Gene symbol
    MAP4K4
  • Short name
    Rabbit Anti- MAP4K4 (pThr187) Polyclonal: PerCP
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- MAP4K4 (pThr187) Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    PerCP
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman mitogen-activated protein kinase kinase kinase kinase 4 (pThr187) polyclonal: PerCP
  • Alternative technique
    polyclonals, rabbit-anti, antibodies-percp, antibodies
  • Alternative to gene target
    mitogen-activated protein kinase kinase kinase kinase 4, FLH21957 and HEL-S-31 and HGK and MEKKK4 and NIK, MAP4K4 and IDBG-63853 and ENSG00000071054 and 9448, transferase activity, Cytoplasm, Map4k4 and IDBG-147835 and ENSMUSG00000026074 and 26921, MAP4K4 and IDBG-630617 and ENSBTAG00000013023 and
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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