K48-linked Tetra-Ubiquitin

Synonyms

K48-linked Tetra-Ubiquitin
Description

Substrate for defined length polyubiquitination
Purity by SDS PAGE

≥95%
Assay

Western Blot
Molecular Weight

34.233 kDa (Band migrates faster on gels)
Storage Temp

-80°C
Shipping

Dry ice
Shelf Life

12 months
Concentration

2.5 mg/ml
Appearance

Liquid
Physical form description

2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.
Background Information

Poly-ubiquitylation of target proteins through linkage at K48, is now the most thoroughly studied of the various chain linkages, and was once considered the hallmark of this post-translational modification. It is now clear that many, if not all, poly-Ub chain topologies likely play distinct and important roles in regulating cellular processes. Nevertheless, K48 linkage remains a critical pathway for the cells to maintain homeostasis through proteolytic degradation, and as such remains very intriguing for the study of DUBs that play a role in the degradation, as well as the proteasome itself. These tetra-ubiquitin chains are generated from the enzymatic linkage (E2-25K) of wild-type ubiquitin through lysine 48. The most distal ubiquitin contains an arginine substitution for a lysine at position 48, limiting the chain length.
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet