anti-WNT-1 Antibody

• Catalog number
  38-176
• Price
  Please ask
• Size
  0.05 mg

• Gene info
  Wnt1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta catenine. Mature human Wnt1 is a glycosylated protein containing 343 amino acid residues.
• Gene names
  INT1, OI15, BMND16, INT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog,
• Antibodie s label
  Biotin
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-1. Human WNT-1 specific antibody was purified by affinity chromatography and then biotinylated.
• Immuno application
  ELISA, Western Blot
• Antibody advice
  ELISA:Direct:To detect hWNT-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.SandwichTo detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1. Western Blot:To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Keep at
  WNT-1 antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
• Works with
  Human
• Omim nr
  139743
• Ncbi nr
  P04628
• Vial with antibody
  Lyophilized
• NCBI GENE nr
  7471
• GENE symbol
  WNT1
• Antigene name
  wingless-type MMTV integration site family, member 1
• Source
  Homo sapiens
• Protein nr
  P04628
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  WNT-1  
• Gene symbol
  WNT1, WNT7A, WNT4, WNT10B, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1
• Short name
  anti-WNT-1 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toWNT-1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:12774
• Gene
  WNT1
• Long gene name
  Wnt family member 1
• Synonyms gene
  • INT1
• Synonyms gene name
  • wingless-type MMTV integration site family, member 1
• GenBank acession
  • X03072
• Locus
  12q13.12
• Discovery year
  2001-06-22
• Entrez gene record
  7471
• Pubmed identfication
  • 2998762
  • 3281802
• Classification
  • Wnt family
• VEGA ID
  OTTHUMG00000170403

Gene info

• Identity
  HGNC:12786
• Gene
  WNT7A
• Long gene name
  Wnt family member 7A
• Synonyms gene name
  • wingless-type MMTV integration site family, member 7A
• Synonyms
  • Wnt-7a
• Synonyms name
  • proto-oncogene Wnt7a protein
• GenBank acession
  • D83175
• Locus
  3p25.1
• Discovery year
  1996-03-12
• Entrez gene record
  7476
• Pubmed identfication
  • 8893824
  • 9161407
• RefSeq identity
  • NM_004625
• Classification
  • Wnt family
• VEGA ID
  OTTHUMG00000129803

Gene info

• Identity
  HGNC:12783
• Gene
  WNT4
• Long gene name
  Wnt family member 4
• Synonyms gene name
  • wingless-type MMTV integration site family, member 4
• Synonyms
  • WNT-4
• GenBank acession
  • AL031281
• Locus
  1p36.12
• Discovery year
  2000-07-31
• Entrez gene record
  54361
• Pubmed identfication
  • 8168088
• Classification
  • Wnt family
• VEGA ID
  OTTHUMG00000002894

Gene info

• Identity
  HGNC:12775
• Gene
  WNT10B
• Long gene name
  Wnt family member 10B
• Synonyms gene name
  • wingless-type MMTV integration site family, member 10B
• Synonyms
  • WNT-12
  • SHFM6
• GenBank acession
  • X97057
• Locus
  12q13.12
• Discovery year
  1997-09-05
• Entrez gene record
  7480
• Pubmed identfication
  • 9121776
  • 9284937
  • 18515319
• RefSeq identity
  • NM_003394
• Classification
  • Wnt family
• VEGA ID
  OTTHUMG00000150734

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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