Mouse anti P53

  • Catalog number
    MUB1500P
  • Price
    Please ask
  • Size
    0,1 mg
  • Category
    Primary Antibodies
  • Long description
    AlteRations of the p53 tumor suppressor gene are considered critical events in multistage carcinogenesis of a wide range of Human cancers. The mutations found in sporadic cancer are commonly mis-sense mutations that inactivate the tumor suppressor activity of p53. These mutant proteins are much more stable than the normal p53 protein, frequently adopt an altered conformation and accumulate within the malignant cells.
  • Antibody come from
    Bp53.12 is a Mouse monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with recombinant Human wild-type p53 protein.
  • Other description
    Each vial contains 100 ug 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
  • Clone
    Bp53.12
  • Antigen antibody binding interaction
    Mouse anti P53 Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    Bp53.12 reacts with a denatuRation-resistant epitope of p53. This antibody is specific for Human p53 and recognizes amino acid sequence 16-25.
  • Research interest
    Cancer, Cell Cycle
  • Application
    Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
  • Antibody s suited for
    Bp53.12 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
  • Storage
    Store at 4°C, or in small aliquots at -20°C.
  • Relevant references
    Bartek, J., Bartkova, J., Vojtesek, B., Staskova, Z., Lukas, J., Rejthar, A., Kovarik, J., Midgley, C. A., Gannon, J. V., and Lane, D. P. (1991). Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of Human malignancies. Oncogene 6, 1699-1703. Bartek, J., Bartkova, J., Lukas, J., Staskova, Z., Vojtesek, B., and Lane, D. P. (1993). Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies. J Pathol 169, 27-34.
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    P53  
  • Gene symbol
    TP53
  • Short name
    Mouse anti P53
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG2a
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to P53
  • Alternative technique
    murine, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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