Protein A Sepharose Column
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Catalog number
6508-1
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Price
Please ask
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Size
1 ml
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Description
Columns with Reusable Protein A conjugated Sepharose beads with ≥16 mg/ml binding capacity & 2.07 ml/min flow rate for antibody purification from multiple sources. Protein A-Sepharose Columns are prepared by covalently coupling recombinant Protein A to 6% cross-linked Sepharose beads. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A. The IgG binding capacity of Protein A-Sepharose Column is ≥ 16 mg human or rabbit IgG per ml of wet beads. Protein A-Sepharose Columns display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
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Product Highlights
•CONTENTS- Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in PBS with 0.02% sodium azide; >5mg Protein A/ml Sepharose beads. - HIGH BINDING CAPACITY: Binding of IgG ≥16 mg human or rabbit IgG/ml Protein A-Sepharose. - MINIMAL LEACHING OF THE LIGAND. - FLOW RATE TESTED*: 2.07 ml/min. *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). - USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
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Storage Temp
+4°C
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Shipping
gel pack
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Shelf Life
12 months
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Notes
For research use only. Not to be used for human or animal treatment or consumption.
MeSH Data
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Name
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Concept
Scope note:
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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