Heme Oxygenase-2 Antibody
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Catalog number3392-100
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PricePlease ask
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Size100 µg
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Antibody TargetHeme Oxygenase-2
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Target Alternative NameHMOX2 , HO-2 ,HO2 , EC 1.14.99.3
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat, monkey, hamster
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Immunogen SequenceSynthetic peptide surrounding amino acid 34 of human HO-2
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Accession2Q32B
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Gene Id149243581
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blotting, Immunoprecipitation, Immunohistochemistry
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Application And UsagesWestern blotting (0.5-4 µg/ml), in immunoprecipitation (20 µg/ml) and Immunohistochemistry (20 µg/ml, frozen section tested). However, the optimal concentrations should be determined individually. The antibody recognizes ~36 kDa Heme-Oxygenase-2 of human, mouse, rat, hamster, and monkey origins. Blocking peptide (Cat.# 3392BP-50) is available separately.
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DescriptionHeme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme, a pro-oxidant, into carbon monoxide and biliverdin. Two heme oxygenase isozymes, termed HO-1 and HO-2, have been identified so far. At the amino acid level, HO-1 and HO-2 are 42% homologous. However, each isozyme is evolutionary highly conserved. HO-1 is the inducible form of the enzyme and is therefore also referred to as stress protein Hsp32. Expression of HO-1 can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO-2 is the constituitive form of the enzyme. It is not inducible by exogeneous stimuli and HO-2 is the most prevalent form in most tissues, except the spleen where HO-1 levels are predominant under normal conditions.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolHMOX2
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Short nameHeme Oxygenase-2 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameHeme Oxygenase-2 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameheme oxygenase 2
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Synonyms gene name
- heme oxygenase (decycling) 2
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Synonyms
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Locus
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Discovery year1992-10-15
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data