Anti-TRAM1 (Polyclonal), ALEXA Fluor 647
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Catalog numberGENTObs-16565R-A647
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 647
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideTRAM1
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SpecificityThis antibody reacts specifically with TRAM1 ALEXA FLUOR® 647
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsCytoplasm
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Antigen SourceKLH conjugated synthetic peptide derived from human TRAM1
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Gene ID23471
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Swiss ProtQ15629
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationStimulatory or required for the translocation of secretory proteins across the ER membrane.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission650nm/665nm
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SynonymsTRAM; PNAS8; TRAMP; Translocating chain-associated membrane protein 1; TRAM1
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Also known asTRAM1 Polyclonal Antibody ALEXA FLUOR® 647
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Other nameAnti-TRAM1 Polyclonal ALEXA FLUOR® 647
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Polyclonal Antibodies made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-TRAM1 ALEXA FLUOR® 647 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.
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ConjugationAlexa Fluor, ALEXA FLUOR 647
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolTRAM1
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Short nameAnti-TRAM1 ALEXA FLUOR® 647
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 647
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Alternative nameTRAM1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nametranslocation associated membrane protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-04-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- TLC domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data