CD247, human recombinant

• Catalog number
  7340-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  T-cell surface glycoprotein CD3 zeta chain, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ
• Alternative_names
  T-cell surface glycoprotein CD3 zeta chain, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ
• Description
  Plays a role in coupling antigen recognition to several intracellular signal-transduction pathways
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Molecular Weight
  15.4 kDa (136 aa, 52-164 aa + His Tag), confirmed by MALDI-TOF.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol and 1 mM DTT.
• Background Information
  T-cell surface glycoprotein CD3 zeta chain, also known as CD247, belongs to the CD3Z/FCER1G family. CD247 is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Recombinant human CD247 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSRVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPR
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CD247  
• Gene symbol
  CD247
• Short name
  CD247, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  CD247 molecule, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  CD247 molecule, CD3-ZETA and CD3H and CD3Q and CD3Z and IMD25 and T3Z and TCRZ, CD247 and IDBG-104547 and ENSG00000198821 and 919, protein homodimerization activity, Plasma membranes, Cd247 and IDBG-202777 and ENSMUSG00000005763 and 12503, CD247 and IDBG-629556 and ENSBTAG00000012700 and 281056

Gene info

• Identity
  HGNC:1677
• Gene
  CD247
• Long gene name
  CD247 molecule
• Synonyms gene
  • CD3Z
• Synonyms gene name
  • CD3z antigen, zeta polypeptide (TiT3 complex)
  • CD247 antigen
• Synonyms
  • CD3H
  • CD3Q
• Synonyms name
  • T-cell surface glycoprotein CD3 zeta chain
• GenBank acession
  • BC025703
• Locus
  1q24.2
• Discovery year
  1989-04-06
• Entrez gene record
  919
• Pubmed identfication
  • 2974162
• RefSeq identity
  • NM_198053
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000034593
• Locus Specific Databases
  • CD247base: Mutation registry for Autosomal recessive CD3Zeta deficiency
  • LRG_36

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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