MEK1/2 (phospho-S218/222) polyclonal antibody

• Catalog number
  BS4733-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2; MAP2K2; MEK-2; MKK2; PRKMK2
• Immunogen
  Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Serine 218.
• Swiss Prot
  Q02750/P36507
• Reactivity
  Human,Mouse,Rat
• Applications
  WB IHC
• Application_all
  WB: 1:500~1:1000 IHC: 1:50~1:200
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  p-MEK1/2 (S218/222) polyclonal antibody detects endogenous levels of MEK-1 protein only when phosphorylated at Ser218. This antibody also detects endogenous levels of MEK-2 protein only when phosphorylated at Thr222.
• Usage
  Western blot (WB) analysis of p-MEK1/2 (S218/222) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate treated with PMA(100nM,30mins) Lane2:Raw264.7 cell lysate treated with PMA(100nM,30mins) Lane3:NIH-3T3 cell lysate treated with PMA(100nM,30mins) Lane4: PC12 cell lysate treated with PMA(100nM,30mins)
• BiowMW
  ~ 43 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  MEK1/2   phospho-S218/222  
• Gene symbol
  MAP2K1
• Short name
  MEK1/2 (phospho-S218/222) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  MEK1/2 (phosphorilated-S218/222) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:6840
• Gene
  MAP2K1
• Long gene name
  mitogen-activated protein kinase kinase 1
• Synonyms gene
  • PRKMK1
• Synonyms
  • MEK1
  • MAPKK1
• GenBank acession
  • L11284
• Locus
  15q22.31
• Discovery year
  1993-11-05
• Entrez gene record
  5604
• Pubmed identfication
  • 9465908
  • 8388392
• Classification
  • Mitogen-activated protein kinase kinases
• VEGA ID
  OTTHUMG00000133196
• Locus Specific Databases
  • The Globin Gene Server
  • LRG_725

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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