CD44, H-CAM, Clone: MAC329, Rat Mouse Monoclonal antibody-Swine, Sheep, Bovine, Goat, Horse, Dog; frozen/paraffin, IH/flow/IP

• Catalog number
  YSRTMCA1449
• Price
  Please ask
• Size
  2 ml

• Alternative name 1
  CD44, H-CAM, Clone: MAC329, Rat Mab anti-Swine, Sheep, Bovine, Goat, Horse, Dog; frozen/paraffin, IH/flow/IP
• Alternative name 2
  CD44, H-CAM, Clone: MAC329, Rat Mab antibody-Swine, Sheep, Bovine, Goat, Horse, Dog; frozen/paraffin, IH/flow/IP
• Alternative name 3
  CD44, H-CAM, Clone: MAC329, Rattus norvegicus Mab antibody-Swine, Ovine, Bovine, Goat, Horse, Canine; frozen/paraffin, IH/flow/IP
• Alternative name 4
  anti-CD44 H-CAM Clone: MAC329 Horse
• Alternative name 5
  CD44 H-CAM Clone: MAC329 Horse
• Clonality
  Monoclonal Antibody
• Description
  This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
• Storage and handling
  Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
• Advisory
  To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
• Properties
  If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Canine or Canis Lupus is mostly Beagle used for drug research. accurate-monoclonals produces ELISA test kits and polyclonal antibodies. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Capra aegagrus hircus, Mus musculus, Rattus norvegicus
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• French translation
  anticorps

• Gene target
  CD44   H-CAM   MAC329   Monoclonal   Swine   frozen/paraffin  
• Gene symbol
  KRIT1, CAMK2N2, NCAPH, CD44-AS1, HLA-H, CFAP251, IGHV3-69-1, CFAP61, FUT1, PIGH
• Short name
  CD44, H-CAM, Clone: MAC329, Mouse Monoclonal antibody-Swine, , , Goat, , ; frozen/paraffin, IH/ /IP
• Technique
  Mouse monoclonal, Antibody, paraffin, frozen, Mouse, Flow, Goat, IP, antibodies against human proteins, antibodies for, goats, Immunoprecipitation, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  Rat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Species
  Dog, Bos Taurus genes are available form the Bovine Genome database. Dogs, Goats, Horses, Mouses, Rats, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  CD44 molecule (Indian blood group), H-CAM, clonality: MAC329, Rat Mouse monoclonal (antibody to-)-swine, ovine, Bovine, caprine, Horse, canine; -25°C frozen/paraffin, IH/flow/IP
• Alternative technique
  antibodies, murine, caprine
• Alternative to gene target
  CD44 molecule (Indian blood group), CDW44 and CSPG8 and ECMR-III and HCELL and HUTCH-I and IN and LHR and MC56 and MDU2 and MDU3 and MIC4 and Pgp1, CD44 and IDBG-39556 and ENSG00000026508 and 960, hyaluronic acid binding, Cell surfaces, Cd44 and IDBG-193505 and ENSMUSG00000005087 and 12505, CD44 and IDBG-636564 and ENSBTAG00000011578 and 281057
• Tissue
  frozen

Gene info

• Identity
  HGNC:1573
• Gene
  KRIT1
• Long gene name
  KRIT1 ankyrin repeat containing
• Synonyms gene
  • CCM1
• Synonyms gene name
  • cerebral cavernous malformations 1
  • KRIT1, ankyrin repeat containing
• Synonyms
  • CAM
• GenBank acession
  • AJ294850
• Locus
  7q21.2
• Discovery year
  1995-06-02
• Entrez gene record
  889
• Pubmed identfication
  • 7604043
  • 11342228
• Classification
  • FERM domain containing
  • Ankyrin repeat domain containing
  • CCM adhesion complex
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000131187
• Locus Specific Databases
  • LRG_650

Gene info

• Identity
  HGNC:24197
• Gene
  CAMK2N2
• Long gene name
  calcium/calmodulin dependent protein kinase II inhibitor 2
• Synonyms
  • CaM-KIIN
• GenBank acession
  • AY037149
• Locus
  3q27.1
• Discovery year
  2005-06-10
• Entrez gene record
  94032
• Pubmed identfication
  • 11444830
  • 9724800
• RefSeq identity
  • NM_033259
• VEGA ID
  OTTHUMG00000156821

Gene info

• Identity
  HGNC:1112
• Gene
  NCAPH
• Long gene name
  non-SMC condensin I complex subunit H
• Synonyms gene
  • BRRN1
• Synonyms gene name
  • barren (Drosophila) homolog
  • barren homolog (Drosophila)
  • barren homolog 1 (Drosophila)
  • non-SMC condensin I complex, subunit H
• Synonyms
  • CAP-H
  • hCAP-H
• GenBank acession
  • BC024211
• Locus
  2q11.2
• Discovery year
  1998-02-11
• Entrez gene record
  23397
• Pubmed identfication
  • 9417923
• RefSeq identity
  • NM_015341
• Classification
  • Condensin I subunits
  • Kleisins
• VEGA ID
  OTTHUMG00000130451

Gene info

• Identity
  HGNC:40133
• Gene
  CD44-AS1
• Long gene name
  CD44 antisense RNA 1
• Synonyms
  • RP1-68D18.4
• Locus
  11p13
• Discovery year
  2017-02-10
• Pubmed identfication
  • 28103885
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:4965
• Gene
  HLA-H
• Long gene name
  major histocompatibility complex, class I, H (pseudogene)
• GenBank acession
  • M31944
• Locus
  6p22.1
• Discovery year
  1991-08-01
• Entrez gene record
  3136
• Pubmed identfication
  • 1517564
  • 2294403
• RefSeq identity
  • NR_001434
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031160
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:28506
• Gene
  CFAP251
• Long gene name
  cilia and flagella associated protein 251
• Synonyms gene
  • WDR66
• Synonyms gene name
  • WD repeat domain 66
• Synonyms
  • MGC33630
  • CaM-IP4
• GenBank acession
  • AL833930
• Locus
  12q24.31
• Discovery year
  2005-05-26
• Entrez gene record
  144406
• Pubmed identfication
  • 17967944
• RefSeq identity
  • NM_144668
• Classification
  • WD repeat domain containing
• VEGA ID
  OTTHUMG00000168948

Gene info

• Identity
  HGNC:5631
• Gene
  IGHV3-69-1
• Long gene name
  immunoglobulin heavy variable 3-69-1 (pseudogene)
• Synonyms gene
  • IGHV3-H
• Synonyms gene name
  • immunoglobulin heavy variable 3-H (provisional)
  • immunoglobulin heavy variable 3-H pseudogene (provisional)
  • immunoglobulin heavy variable 3-H (pseudogene)
• Synonyms
  • IGHV3-h
• GenBank acession
  • AJ879484
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28402
• Pubmed identfication
  • 16133446
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191145

Gene info

• Identity
  HGNC:15872
• Gene
  CFAP61
• Long gene name
  cilia and flagella associated protein 61
• Synonyms gene
  • C20orf26
• Synonyms gene name
  • chromosome 20 open reading frame 26
• Synonyms
  • DKFZP434K156
  • dJ1002M8.3
  • CaM-IP3
• Synonyms name
  • hypothetical protein LOC26074
• GenBank acession
  • AL117439
• Locus
  20p11.23
• Discovery year
  2001-06-21
• Entrez gene record
  26074
• Pubmed identfication
  • 11780052
  • 17967944
• RefSeq identity
  • NM_015585
• Classification
  • Cilia and flagella associated
• VEGA ID
  OTTHUMG00000032006

Gene info

• Identity
  HGNC:4012
• Gene
  FUT1
• Long gene name
  fucosyltransferase 1 (H blood group)
• Synonyms gene
  • H
  • HSC
• Synonyms gene name
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
• Synonyms name
  • galactoside 2-alpha-L-fucosyltransferase
• Locus
  19q13.33
• Discovery year
  1989-06-06
• Entrez gene record
  2523
• RefSeq identity
  • NM_000148
• Classification
  • Fucosyltransferases
  • Blood group antigens
• VEGA ID
  OTTHUMG00000183325
• Locus Specific Databases
  • Blood Group Antigen Mutation Database
  • LRG_810

Gene info

• Identity
  HGNC:8964
• Gene
  PIGH
• Long gene name
  phosphatidylinositol glycan anchor biosynthesis class H
• Synonyms gene name
  • phosphatidylinositol glycan, class H
• Synonyms
  • GPI-H
• Synonyms name
  • phosphatidylinositol N-acetylglucosaminyltransferase subunit
• Locus
  14q24.1
• Discovery year
  1994-09-06
• Entrez gene record
  5283
• Pubmed identfication
  • 8204896
• RefSeq identity
  • NM_004569
• Classification
  • Phosphatidylinositol glycan anchor biosynthesis
• VEGA ID
  OTTHUMG00000171806

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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