BrdU Reagent for in vivo injection
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Catalog number
X2834
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Price
Please ask
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Size
5 x 5 mg
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Category
Other Reagents
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Long description
Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. The molecular weight of BrdU is 307.1.
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Antibody come from
n/a
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Other description
Provided as sterile filtered solution in phosphate buffered saline
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Clone
not specified
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Antigen antibody binding interaction
BrdU Reagent for in vivo injection
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Antibody is raised in
see techfile
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
Cell Biology
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Application
Immunostaining
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Antibody s suited for
Prior to BrdU immunostaining to detect proliferating cells it is necessary to label the cells or tissue with BrdU. Labeling can either be done in vitro for cell cultures or performed in vivo for experimental animals. Both protocols are provided: In vivo labeling of mouse cells with BrdU Two common methods used for in vivo BrdU labeling of tissues and cells include the intraperitoneal injection of a BrdU-containing solution into mice and the feeding of mice with BrdU that is added to their drinking water. a) Intraperitoneal Method: A 10 mg/ml solution of BrdU in sterile 1X DPBS is provided for in vivo use. Inject mice i.p. with 100-200 μl (1-2 mg) of BrdU solution. Incorporation of BrdU can be detected in thymus and bone marrow in as little as 1 hr post injection. 24 hrs post injection BrdU can be detected in most of the tissues. b) Drinking water method: Dilute BrdU to 0.8 mg/ml in the drinking water. The BrdU mixture should be made up freshly and changed daily. Prolonged feeding of BrdU can have toxic effects. Some investigators have reported lethal effects associated with 14 days of continuous BrdU feeding. For longer term studies, some investigators have reported that feeding mice with BrdU for 9 consecutive days followed by a change over to normal water has worked effectively. BrdU incorporation by cells from these animals has been detected past 70 days.
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Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles. Products are stable for one year from purchase when stored properly
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
For research use only. Not for use in human diagnostics or therapeutics.
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