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Synonyms
Calpain
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Alternative_names
Calcium-activated neutral proteinase 2, Short name=CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, Short name=M-calpain
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Description
A cysteine protease involved in apoptosis activation
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Recombinant
No
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Source
Human Erythrocyte
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Activity Specifications test method
>1000 units/mg protein
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Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze one pmol Suc-LLVY-AMC per minute at 25 °C.
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Molecular Weight
112.0 kDa
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Storage Temp
-70°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Liquid
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Physical form description
In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
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Background Information
Ca²ᶧ-dependent cysteine proteinase with low Ca²ᶧrequirement (half maximal activation = 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin. Involves in apoptosis activation.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.