Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit

• Catalog number
  K541-30
• Price
  Please ask
• Size
  30 ml isolation

• Description
  Simple, one step assay to isolate PBMC
• Summary
  • Isolation of PBMCs from human whole blood. • Determination of cell viability and also contamination from RBCs in isolated cell fraction.
• Species Reactivity
  Human
• Applications
  • Isolation of PBMCs from human whole blood. • Determination of cell viability and also contamination from RBCs in isolated cell fraction.
• Sample Type
  Human whole blood (collected within 12 hr)
• Features Benefits
  • PBMC high yields (≥2.5 x106 cells/ml) • By using the Viability Stain, it was proven that 99% of the cells are viable and the isolated fraction contains low red blood cell counts (≤ 3%)
• Storage Conditions
  4 °C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Peripheral blood mononuclear cells (PBMCs) are found in the peripheral blood circulating throughout the body and not sequestered in the lymphatic system, spleen, liver or bone marrow. PBMCs are composed of four cell types: T cells, B cells, Natural Killer cells and monocytes, each having a round nucleus. Applicable research areas include: stem cell research, evaluation the immunotherapeutic potential of T or B cells and evaluation of inflammatory response orchestrated by cytokines and chemokines. Based on the unique density of each component, the whole blood is separated into plasma, PBMCs, density gradient media and red blood cells (RBCs). BioVision’s Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit for PBMC Isolation is the first kit available to provide a complete set of components for the isolation of PBMCs from human blood and also allow visualization of viable cells. High yields of PBMCs (≥2.5 x106 cells/ml) have been obtained following this simple and time-saving protocol. By using the Viability Stain, it was proven that 99% of the cells are viable and the isolated fraction contains low red blood cell counts (≤ 3%).
• Data Sheet Link
• Additional description
  Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. Biovision supplies this blood related product in 1. For cells, cell lines and tissues in culture till half confluency.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  Peripheral   Mononuclear   Isolation   Viability   Kit  
• Short name
  Peripheral Blood Mononuclear Isolation Viability Kit
• Technique
  blood, blood samples
• Species
  Human, Humans
• Alternative name
  H. sapiens Peripheral Blood Mononuclear cellular purification and cytotoxity reagent
• Alternative technique
  sera, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  blood, cell

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