Human Recombinant UCHL1

• Catalog number
  6306-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant UCHL1
• Alternative_names
  Ubiquitin carboxyl-terminal esterase L1, UCHL1, PARK5, Uch-L1, PGP9.5.
• Description
  A deubiquitinating enzyme
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 ug of protein (determined by LAL method)
• Biological activity
  > 300 pmole/min/ug
• Molecular Weight
  24.8 kDa (223 aa)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA.
• Background Information
  PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UCHL1  
• Gene symbol
  UCHL1-DT, UCHL1
• Short name
  Recombinant UCHL1
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. UCHL1
• Alternative technique
  rec

Gene info

• Identity
  HGNC:40600
• Gene
  UCHL1-DT
• Long gene name
  UCHL1 divergent transcript
• Synonyms gene
  • UCHL1-AS1
• Synonyms gene name
  • UCHL1 antisense RNA 1 (head to head)
• GenBank acession
  • DB169394
  • CN277289
• Locus
  4p13
• Discovery year
  2012-12-01
• Entrez gene record
  101410542
• RefSeq identity
  • NR_102709
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000160405

Gene info

• Identity
  HGNC:12513
• Gene
  UCHL1
• Long gene name
  ubiquitin C-terminal hydrolase L1
• Synonyms gene
  • PARK5
• Synonyms gene name
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Synonyms
  • PGP9.5
  • Uch-L1
• Synonyms name
  • ubiquitin thiolesterase
• GenBank acession
  • BC000332
• Locus
  4p13
• Discovery year
  1991-07-15
• Entrez gene record
  7345
• Pubmed identfication
  • 1840236
• RefSeq identity
  • NM_004181
• Classification
  • Ubiquitin C-terminal hydrolases
• VEGA ID
  OTTHUMG00000099377

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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