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Synonyms
SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15
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Alternative_names
SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15
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Description
Involved in sperm-egg adhesion
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
≥92%
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Assay
SDS-PAGE
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Endotoxin Level
< 0.1 ng/ug of protein (
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Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 52.1 kDa. The predicted N-terminus is Leu 36. DTT-reduced Protein migrates as 64-66 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.0-7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
SPAM1
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Short name
CellExp™ SPAM1, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), HEL-S-96n and HYA1 and HYAL1 and HYAL3 and HYAL5 and PH-20 and PH20 and SPAG15, SPAM1 and IDBG-39033 and ENSG00000106304 and 6677, hyalurononglucosaminidase activity, Plasma membranes
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