Lysosomal Intracellular Activity Assay Kit

• Catalog number
  K448-50
• Price
  Please ask
• Size
  50 assays

• Summary
  • Detection method- Flow cytometry and Fluorescence Microscopy • Species reactivity- Mammalian • Applications- - Measurement of Intracellular lysosomal activity - Screening compounds with anti-lysosomal activity
• Detection Method
  Flow Cytometry (FL1)/ Microscopy (Ex: 488 nm)
• Species Reactivity
  Mammalian
• Applications
  • Measurement of lysosomal intracellular activity. • Elucidation of the mechanisms of endocytic pathway in living cells. • Screening compounds with anti-lysosomal intracellular activity
• Sample Type
  Suspension or adherent cell cultures
• Features Benefits
  Measurement of lysosomal intracellular activity.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Lysosomes are membrane-bound organelles important for various cellular processes. They contain hydrolytic enzymes that are utilized in the metabolism of some biomolecules. The extracellular cargo (e.g. nutrients toxins) binds to the cell membrane and is subsequently transported into membrane-bound endosomes for further degradation by lysosomes while intracellular components are transported to lysosomes through autophagy. Lysosomal dysfunction is associated with many human conditions such as aging and neurodegenerative disease. Although the intracellular activity of lysosome is difficult to measure in living cells, BioVision has developed a proprietary Lysosome-Specific Self-Quenched Substrate which has low background fluorescence, high signal to background ratio and is pH insensitive. The substrate, acting as endocytic cargo, can be taken up by cells and degraded within an endo-lysosomal vesicle. The fluorescent signal is recovered from the Self-Quenched Substrate. The fluorescence signal, generated by degradation, is proportional to the intracellular lysosomal activity and can be measured using a fluorescence microscopy and/or flow cytometry. Lysosomal Intracellular Activity Assay Kit (Cell-Based) includes Cytochalasin D, a cell-permeable inhibitor of endocytosis that serves as an experimental control. This easy-to-use, non-radioactive kit allows imaging and accurate measurement of de-quenching substrate in cultured cells
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Lysosomal   Intracellular   Activity   Kit  
• Short name
  Lysosomal Intracellular Activity Assay Kit
• Technique
  intracellular, Assay, assays
• Host
  Assay
• Alternative name
  Lysosomal Intracellular Activity test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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