Human Recombinant PPM1G

• Catalog number
  6369-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Human Recombinant PPM1G
• Alternative_names
  Protein phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2CGAMMA.
• Description
  Negative regulator of cell stress response pathways
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥85%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity: >5,000 units/mg.
• Results
  >5,000 units/mg
• Unit Definition
  One unit will hydrolyze 1 nmole of p-nitrophenylphosphatate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.
• Molecular Weight
  61.4 kDa (566 aa, 1-546 aa + NT His-Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT, 0.1 M NaCl, and 0.1 mM PMSF.
• Background Information
  PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PPM1G  
• Gene symbol
  PPM1G
• Short name
  Recombinant PPM1G
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. PPM1G
• Alternative technique
  rec

Gene info

• Identity
  HGNC:9278
• Gene
  PPM1G
• Long gene name
  protein phosphatase, Mg2+/Mn2+ dependent 1G
• Synonyms gene name
  • protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
  • protein phosphatase, Mg2+/Mn2+ dependent, 1G
• Synonyms
  • PP2CG
  • PP2Cgamma
• Synonyms name
  • PP2C, gamma
  • protein phosphatase 2C, gamma isoform
• GenBank acession
  • Y13936
• Locus
  2p23.3
• Discovery year
  1997-11-11
• Entrez gene record
  5496
• Pubmed identfication
  • 9276438
• RefSeq identity
  • NM_002707
• Classification
  • Protein phosphatases, Mg2+/Mn2+ dependent
• VEGA ID
  OTTHUMG00000097788

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products