SMAD2/3 Antibody

• Catalog number
  R30257
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  SMAD2/3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the SMAD2/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This SMAD2/3 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q15796
• Purity
  Antigen affinity
• Description
  SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Transforming growth factor (TGF)-beta stimulation leads to phosphorylation and activation of Smad2 and Smad3, which form complexes with Smad4 that accumulate in the nucleus and regulate transcription of target genes. Smad2 and Smad3 share highly homology. SMAD2/3 signal transduction appeared to be important in the regulation of muscle-specific genes. SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development.
• Immunogen
  An amino acid sequence from the C-terminus of human SMAD2/3 (QSPNCNQRYGWHPATVC) was used as the immunogen for this SMAD2/3 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the SMAD2/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SMAD2/3  
• Gene symbol
  SMAD2
• Short name
  Anti-SMAD2/3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to SMAD2/3
• Alternative technique
  antibodies
• Alternative to gene target
  SMAD family member 2, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, transforming growth factor beta receptor, nuclei, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125,516010

Gene info

• Identity
  HGNC:6768
• Gene
  SMAD2
• Long gene name
  SMAD family member 2
• Synonyms gene
  • MADH2
• Synonyms gene name
  • MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2 (Drosophila)
• Synonyms
  • MADR2
  • JV18-1
• GenBank acession
  • U65019
• Locus
  18q21.1
• Discovery year
  1996-11-15
• Entrez gene record
  4087
• Pubmed identfication
  • 8752209
  • 8673135
• RefSeq identity
  • NM_005901
• Classification
  • SMAD family
• VEGA ID
  OTTHUMG00000132652

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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