ARFGAP1 Antibody

• Catalog number
  R32624
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  ARFGAP1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  Optimal dilution of the ARFGAP1 antibody should be determined by the researcher.
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q8N6T3
• Purity
  Antigen affinity
• Description
  ADP-ribosylation factor GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARFGAP1 gene. And this gene is mapped to 20q13.33. The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
• Immunogen
  A human recombinant protein corresponding to amino acids M1-Y183 was used as the immunogen for the ARFGAP1 antibody.
• Storage
  After reconstitution, the ARFGAP1 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ARFGAP1  
• Gene symbol
  ARFGAP3, ARFGAP1
• Short name
  Anti- ARFGAP1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to ARFGAP1
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:661
• Gene
  ARFGAP3
• Long gene name
  ADP ribosylation factor GTPase activating protein 3
• Synonyms gene
  • ARFGAP1
• Synonyms gene name
  • ADP-ribosylation factor GTPase activating protein 1
• GenBank acession
  • AK002083
• Locus
  22q13.2
• Discovery year
  1999-11-19
• Entrez gene record
  26286
• Pubmed identfication
  • 10704287
  • 11172815
• RefSeq identity
  • NM_014570
• Classification
  • ArfGAPs
• VEGA ID
  OTTHUMG00000150718

Gene info

• Identity
  HGNC:15852
• Gene
  ARFGAP1
• Long gene name
  ADP ribosylation factor GTPase activating protein 1
• Synonyms gene
  • ARF1GAP
• Synonyms gene name
  • ADP-ribosylation factor 1 GTPase activating protein
• Synonyms
  • FLJ10767
  • bA261N11.3
• GenBank acession
  • AK001629
• Locus
  20q13.33
• Discovery year
  2001-06-21
• Entrez gene record
  55738
• Pubmed identfication
  • 11210549
• RefSeq identity
  • NM_018209
• Classification
  • ArfGAPs
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032965

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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