Anti-Mouse IgG2a-Biotin Conjugate Polyclonal antibody
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Catalog number
83503
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Price
Please ask
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Size
1mg
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Clonality
Polyclonal antibody
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Long name
Polyclonal antibody against Mouse IgG2a-Biotin Conjugate
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Aplication
You will receive the antybody in PBS(7,4 pH), for future delutions PBS or mediun similar to the one used for the assay are suitable.
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Antigen
Mouse IgG2a-Biotin Conjugate
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Storage
Could be stored for at least 12 month in frozen condition at -70C.It is recomendable to prepare aliquotes in order to avoid repetable freezing and thawing as they may denaturate the peptide chains/bonds in the antibodies and reduce/change the activity of the antibody. Prior to use briefly centrifuge the vial to collect its content on the bottom.
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Product description
Anti-Mouse IgG2a-Biotin Conjugate Polyclonal antibody can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by QED Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Short name
Mouse IgG2a-Biotin Conjugate pAb
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Technique
Polyclonal, Antibody, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
mouse
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Isotype
IgG2a
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Label
Biotin
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Species
Mouse, Mouses
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Alternative name
Antibody toMouse IgG2a-biotinilated Conjugate polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies, murine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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